Talk:Polyhistidine-tag

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This article is substantially duplicated by a piece in an external publication. Please do not flag this article as a copyright violation of the following source:

Surhone, L. M., Timpledon, M. T., & Marseken, S. F. (2010), Protein methods: Protein, Site-Directed Mutagenesis, Genetic Code, Green fluorescent Protein, His-Tag, BLAST, Genetic Linkage, Betascript PublishingCS1 maint: multiple names: authors list (link) Click the "show" link above for further details.
OCLC 694818613, ISBN 9786130495107.

Original text:

A his-tag is a technique used in biochemistry to purify proteins. The protein to purify is expressed as a fusion protein, carrrying a series of histidine residues at either the amino or the carboxyl end. The name-giving histidine residues will stick to a nickel surface, and can be eluted from there via imidazol. His-tagged proteins can also be identified on a Western blot via α-his-antibodies.

dekay 11:13, 18 Aug 2004 (UTC)==Generic name== I've revised this to say "polyhistidine-tag", which is apparently the correct generic name, rather than "his-tag", which EMD Biosciences are claiming is a trademark. This isn't a topic I know much about, so please let me know on my talk page if there is any problem with this change. Angela . 21:42, 15 October 2005 (UTC)

Generic name?[edit]

User Angela moved the his tag article to polyhistidine tag, because of a trademark issue. I have written several parts of the original article and I don't agree that a his-tag is the same as the poly histidine tag. The purification and detection methods that described in this article work for the his tag, but I am not whether everything can transferred to the poly histidine tag. The copy paste edit from Angela did not improve this article, but made it rather confusing by mixing things up.
The term his-tag is the most frequently used term in scientific literature and the term poly histidine tag is used less frequent.

The trademark name for the His tag is 6xHis tag (Qiagen, which holds an exclusive licence for parts of the technology)

Poly histidine tag is a copy cat approach of the his tag and is a possible infringment of the original intelectual property owned by Roche.
his tag or better the 6xhis tag is not well discribed by the term poly histidine tag becausethe latin prefix poly leterally means many which is not the case for six histitdines the appropriate word might be oligo his tag (but that is not currently used in scientific literature)

JOK 11:38, 25 October 2005 (UTC)

Please fix whatever factual issues there are here and explain whatever minor differences there may be between the generic and non-generic versions. Here is the email that the foundation just got from EMD:

 The "Polyhistidine-tag" entry provided by your link below is fine, however   I note that "His Tag" (without a hyphen) is still listed as an entry in   your encyclopedia.  Furthermore, after discussing this issue internally, we   are now agreeable to have Wikipedia refer to our trademark (His-Tag®), as   long as Wikipedia makes it clear that this trademark is owned by EMD   Biosciences.      Therefore I would like to ask that you remove the His Tag entry from your   encyclopedia and transfer any pertinent information to the   Polyhistidine-tag entry, making sure that any remaining reference to   His-Tag clearly indicate that this registered trademark belongs to EMD   Biosciences.     I look forward to your reply and resolving this issue to mutual   satisfaction.      Regards,   Marie      Marie Azzaria, Ph.D.   Manager, Intellectual Property

The way things are now is fine. I don't want to deal with this issue again. Recreating the separate article at the registered trademark name may put the foundation in needless legal jeopardy. So please don't do that. --mav 03:39, 29 October 2005 (UTC)

Does this article really need a list a of manufacturers? I can see why it mentions the labs that first developed it, but this is an encyclopaedia, not a trade directory :s Abergabe (talk) 08:35, 27 August 2010 (UTC)

pure protein from bacterial lysate running just a Ni-Column?[edit]

Prokaryotes have a ton of histidine rich proteins that also stick to Ni-Columns. I don't think it's fair to imply that you can do a one step prep (from E.Coli for instance) using just a His Tag. I'm happy to be corrected, but it's going to take a citation of some sort to convince me. Or at the very least a nice looking gel. I(q) = User(q)·Talk(q) 12:10, 1 September 2011 (UTC)

Oops, just noticed the section a few paragraphs down on well known contaminants. Guess that sufficiently addresses my concerns. I(q) = User(q)·Talk(q) 12:14, 1 September 2011 (UTC)

I wont claim that I ever did a one-step purification but by excess washing with buffers containing ~10-20 mM imidazole and excess of target protein you can end up with pure protein (no detectable contaminants on Coomassie stained PAGE). --09:18, 22 April 2013 (UTC) — Preceding unsigned comment added by JayPP (talk • contribs)

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